What we do

Solutions for complex biological challenges

We are the leading provider in genomics, transcriptomics, proteomics, microbiome and single cell analysis.

How we do it

We have expertise in a wide range of commissioned services and research projects

We custom design our services to your needs. We have more than two decades of knowledge and experience in nucleic acid analysis and sample preparation.

We optimize entire workflows by assessing sample quality, validating performance, and analyzing single or multiple biomarkers with the best technology, including our own. We also support data analysis and modeling.

Technologies & Applications

Expert in nucleic acid sample preparation and analyses

We have significant experience working with a wide range of challenging sample types, from a variety of organisms. We offer complete analysis packages – spanning project planning to qPCR data analysis – as well as execution of discrete project parts that require expertise and/or instrumentation. We can perform routine assignments for optimized workflow, or develop new methods optimized for your own experiments.

Our Services

Technology & Applications
Genomics & Epigenomics
qPCR, dPCR

Assay design, transfer, optimization, validation

Genotyping

SNP profiling

Rare mutation

Residual DNA

Copy number variation

Absolute quantification

Our services allow a variety of applications for analysis of the genome. We offer SNP profiling and rare mutation detection, copy number variation analysis, whole genome sequencing, de novo sequencing, whole exome sequencing, targeted sequencing, amplicon sequencing), and epigenome studies (Chromatin IP (ChIP) sequencing, methylation analysis).

NGS

Whole genome sequencing (WGS)

16S and ITS Sequencing

Amplicon sequencing

Exome sequencing

Chromatin IP (ChIP) sequencing

Methylation analysis

Hybridisation capture

Our services allow a variety of applications for analysis of the genome. We offer SNP profiling and rare mutation detection, copy number variation analysis, whole genome sequencing, de novo sequencing, whole exome sequencing, targeted sequencing, amplicon sequencing), and epigenome studies (Chromatin IP (ChIP) sequencing, methylation analysis).

Transcriptomics
qPCR, dPCR

Assay design, transfer, optimization, validation

Single cell

Gene expression

Pharmacodynamics

We offer several technologies and platforms for studying gene expression, from single genes to full transcriptomes, including miRNA. We study the transcriptome using qPCR, dPCR, and NGS, and offer a variety of unique technologies and platforms.

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Two Tailed RT-qPCR

miRNA profiling

Genotyping

Customized assays and solutions to address your particular challenge

TATAA Biocenter researchers invented Two-Tailed RT-qPCR in 2017 (Androvic et al., 2017), which offers a superior solution for miRNA profiling. Instead of using a single binding probe, it uses two hemiprobes connected by a folded tether. Each hemiprobe binds to different stretches of the same microRNA. Binding is exceedingly specific, as the impact of a mismatch is much more profound in a short hemiprobe. The cDNA formed can then be PCR amplified using two sequence specific primers. SYBR or hydrolysis probes can be used for detection. High melting resolution analysis can be used for non-specific products detection.

NGS

Small RNA Sequencing

mRNA Sequencing

Full transcriptome

Gene Expression Profiling by 3’mRNA

Single cell

Extraction-free miRNA analysis

Send us your pre-prepared libraries for sequencing or combine NGS sequencing with our other services (e.g., nucleic acid extraction, experimental design, advanced analysis, etc.) for a complete workflow.

Proteomics
Olink (qPCR and NGS)

Protein expression profiling

Our Olink Proteomics platforms generate high quality data even in highly multiplexed formats and their applications are enormous for powerful analysis of multivariate biomarker patterns.

Our Olink-qPCR based platform offers protein expression for disease-specific or biological pathways. Our Olink Explore 1536/384 platform measures 1,536 proteins with PEA technology and readout on NGS. One single system can perform1.3 million protein measurements per week. And requires less than 3µL sample to screen the entire library!

Technology & Applications
Microbiome
qPCR, dPCR

Viability PCR

Pathogen detection

The number of viable and non-viable cells in a solution are quantified using an optimized workflow based on digital PCR.

NGS

16S rRNA sequencing

Our 16S rRNA sequencing offering allows for identification, classification and quantification of microbes and bacteria within complex biological mixtures

Gene Therapy
qPCR, dPCR

Drug exposure, biodistribution and shedding

mRNA quantitation and cleavage

siRNA PK-PD

NGS

Degradome analysis

Off-target effect

Biomolecules
Robotic liquid handling

Challenging samples

High-throughput aliquoting

Using our Hamilton STAR and Nimbus robotic systems.

Extraction

Genomic DNA, cf-DNA, total RNA, smallRNAs, cf-RNA, exosomes

Automated or manual

TATAA successfully processes sample types derived from a variety of organisms and tissues, including: yeast, plants, bacteria, human and animal tissues, cell pellets, cell cultures, cartilage pellets, bone, tumors, biopsies, saliva, formalin fixed paraffin embedded (FFPE) tissues, cervical samples, LMD samples, stem cells, single cells, process samples for residual DNA, etc

Processing

Quantity and quality testing of RNA, miRNA, DNA, and proteins

Normalization and cDNA synthesis

Library preparation and quality control

We have access to many methods and instruments for quantifying biomolecule amounts and testing samples for quality, integrity and purity. This provides the highest quality and reproducibility of protocols, which are essential for any downstream processing and analyses. Our systems require minute sample volumes (less than 2 µL), allowing you to maximize samples for further analyses. Our automated gel electrophoresis platforms make your analyses fast and highly cost efficient. Nucleic acids can also be quantified using NanoDrop ND-1000 as well as PicoGreen. RNA must be converted into cDNA with reverse transcription (RT) for real-time PCR analysis. Our experts, who laid the ground for all optimization protocols of reverse transcription (Ståhlberg, et al., 2004), will provide you with the best method possible. To avoid variations in the amounts of sample used for the RT, a normalization of the RNA concentrations should be performed before the cDNA synthesis.

Biostatistics & Informatics

Integration and analysis of NGS and proteomics data

TATAA Biocenter can support your data analysis and help getting the most out of your data. Our expert statisticians can help identifying the most significant and distinctive reporter genes, the most stable reference genes, compare expression of genes in groups, test the significance of treatment, distinguish sample types and groups of genes based on multivariate expression profiles, and develop classification algorithms based on training samples to classify patient samples.

Additional Services

Validation of kits, reagents and instruments

Direct Blood Genotyping

Single cell profiling

Cell-free DNA analysis

Analysis of circulating tumor cells 

Residual DNA analysis

Consulting

Experimental design

Sample collection and processing workflow

Customized solutions to address your particular challenge

Workflow

From sampling to results

Optimization and validation of laboratory analysis is important but not sufficient to ensure high quality results. Lippi et al (2011) estimated that 60% to 70% of analytical problems can be attributed to mishandling procedures during sample collection, transport, preparation and storage. We advise on sampling procedures including sampling tubes, handling of samples, transport and storage conditions, as well as quality assessment of representative samples.

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TATAA’s Unique Offerings

Experiment design and optimization

We help you design your experiments and optimize setup to reduce costs and improve power.

Proper experiment design can save you substantial time and money. Before starting any large study, you should perform a small pilot to estimate variance contributions from the different experimental. Defective steps can be identified and improved. Once all steps are optimized, you can best arrange the nesting of the experiment by determining at which steps to perform technical replicates to obtain the highest data quality within your budget.

Once a standard operating procedure is in place, you can estimate the number of subjects to be recruited for the study based on the total technical variance of the experiment.

Why Choose TATAA?
Custom Solutions

Expert advice and consulting

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Consulting and advice

We provide consulting and advice on sampling, sample extraction, storage, transportation, experiment design, instruments, reagents and methods, primer design, data analysis and statistics. We can also help you with all your nucleic acid analyses needs.

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Priority consulting

Customers with an active consulting account benefit from timely and cost-effective support for real-time PCR-related questions. As a Priority Customer you can contact us whenever you need support, and we will put you in contact with the scientist at TATAA that can best answer your question.

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Sample quality matters.

Pre-analytical steps are so pivotal that the European Union sponsored the SPIDIA/SPIDIA4P initiative to standardize and improve the generic pre-analytical tools and procedures for in vitro diagnostics. TATAA has been a member of this consortium since 2008.

Molecular in vitro diagnostics have enabled significant progress in medicine. Further progress is expected through new technologies that analyze signatures of nucleic acids, proteins, and metabolites in human tissues and body fluids. However, molecular profiles can change drastically during primary sample collection, transport, storage, and processing – jeopardizing the reliability of diagnostic outcomes and research.

Therefore, standardizing the entire diagnostic workflow, from sample collection to analytical measurement, is needed to ensure reproducibility of results and improve clinical decision-making and healthcare.